Journal: Circulation: Cardiovascular Imaging

Article Title: Molecular Imaging of VWF (von Willebrand Factor) and Platelet Adhesion in Postischemic Impaired Microvascular Reflow

doi: 10.1161/circimaging.118.007913

Figure Lengend Snippet: Figure 1. Mean (±SEM) microsphere- derived risk area from animals assigned to either (A) 30 min or (B) 45 min of coronary occlusion. C, Mean (±SEM) microvascular blood flow from myocardial contrast echocardiography (MCE) performed during coronary occlusion in the risk area and remote territories, as well as from sham-treated mice. D, Example of background-subtracted, color-coded MCE images during coronary occlusion, and time-intensity data after a destructive pulse sequence from the risk area and remote territory. For images, time after destructive pulse is at upper left and color-coded scale at bottom of the 5 s image. ADAMTS13 indicates a disintegrin and metalloprotein- ase with a thrombospondin type-1 motif member 13; LV, left ventricle; RA, risk area; and WT, wild-type. *P <0.05 vs remote territory.

Article Snippet: In Vivo Imaging Study Design Protocol 1 Closed-chest myocardial IR with 30 minutes of transient left anterior descending coronary artery (LAD) occlusion was performed in WT mice (n=40), WT mice treated with recombinant full-length human ADAMTS13, 5 μg IV (R&D Systems, Minneapolis, MN) just before IR (n=14), and ADAMTS13−/− mice (n=16).

Techniques: Derivative Assay, Sequencing

Journal: Circulation: Cardiovascular Imaging

Article Title: Molecular Imaging of VWF (von Willebrand Factor) and Platelet Adhesion in Postischemic Impaired Microvascular Reflow

doi: 10.1161/circimaging.118.007913

Figure Lengend Snippet: Figure 2. Postischemic myocardial perfusion by MCE in mice undergo- ing 30 min of ischemia and reperfusion measured in the remote region and the risk area. Data (mean±SEM) are shown for (A) myocardial microvascular blood flow (MBF), (B) microvascular blood volume (MBV), and (C) microvascular flux rate (β). ADAMTS13 indicates a disintegrin and metalloproteinase with a thrombo- spondin type-1 motif member 13. *P <0.05 vs remote territory; †P <0.05 vs WT (wild type); ‡P <0.05 only before correction for multiple comparison.

Article Snippet: In Vivo Imaging Study Design Protocol 1 Closed-chest myocardial IR with 30 minutes of transient left anterior descending coronary artery (LAD) occlusion was performed in WT mice (n=40), WT mice treated with recombinant full-length human ADAMTS13, 5 μg IV (R&D Systems, Minneapolis, MN) just before IR (n=14), and ADAMTS13−/− mice (n=16).

Techniques: Comparison

Journal: Circulation: Cardiovascular Imaging

Article Title: Molecular Imaging of VWF (von Willebrand Factor) and Platelet Adhesion in Postischemic Impaired Microvascular Reflow

doi: 10.1161/circimaging.118.007913

Figure Lengend Snippet: Figure 3. Mean (±SEM) signal enhance- ment on MCE molecular imaging after 30 min of ischemia and reperfusion for (A) VWF (von Willebrand factor) A-1 domain and (B) platelet GP (glycoprotein) Ibɑ. Data are expressed as signal difference between targeted and control microbubbles (MB). C, Examples of color-coded MCE molecular imaging in the short-axis plane for targeted and control MBs (color scale at bottom) and the microsphere-derived risk area in the same short-axis plane. *P <0.05 vs remote territory; †P <0.05 vs wild-type and ADAMTS13−/− (a disintegrin and metal- loproteinase with a thrombospondin type-1 motif member 13); ‡P <0.05 vs WT (wild type).

Article Snippet: In Vivo Imaging Study Design Protocol 1 Closed-chest myocardial IR with 30 minutes of transient left anterior descending coronary artery (LAD) occlusion was performed in WT mice (n=40), WT mice treated with recombinant full-length human ADAMTS13, 5 μg IV (R&D Systems, Minneapolis, MN) just before IR (n=14), and ADAMTS13−/− mice (n=16).

Techniques: Imaging, Control, Derivative Assay

Journal: Circulation: Cardiovascular Imaging

Article Title: Molecular Imaging of VWF (von Willebrand Factor) and Platelet Adhesion in Postischemic Impaired Microvascular Reflow

doi: 10.1161/circimaging.118.007913

Figure Lengend Snippet: Figure 5. Mean (±SEM) infarct size by triphenyltetrazolium chloride staining as a percentage of the total microsphere-de- rived risk area for mice undergoing either (A) 30 min of ischemia followed by 90 min of reperfusion or (B) 45 min of ischemia followed by 3 d of reperfusion. *P <0.05 vs WT (wild type) before Bonfer- roni correction; †P <0.05 vs WT+ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type-1 motif member 13) after Bonferroni correction.

Article Snippet: In Vivo Imaging Study Design Protocol 1 Closed-chest myocardial IR with 30 minutes of transient left anterior descending coronary artery (LAD) occlusion was performed in WT mice (n=40), WT mice treated with recombinant full-length human ADAMTS13, 5 μg IV (R&D Systems, Minneapolis, MN) just before IR (n=14), and ADAMTS13−/− mice (n=16).

Techniques: Staining

Journal: Circulation: Cardiovascular Imaging

Article Title: Molecular Imaging of VWF (von Willebrand Factor) and Platelet Adhesion in Postischemic Impaired Microvascular Reflow

doi: 10.1161/circimaging.118.007913

Figure Lengend Snippet: Figure 4. Examples of immunohistochemis- try from the sham-treated control WT (wild type) mice (anterior and posterior myocar- dial with anterior labeled as risk area) and from postischemic WT and ADAMTS13−/− (a disintegrin and metalloproteinase with a thrombospondin type-1 motif member 13) mice. The top and bottom rows show fused im- ages for platelet CD41 immunostaining (red), endothelial lectin staining (green), and nuclear counterstain (blue). The middle row shows corresponding images for only the red channel (platelet CD41) in the risk area. For the insets, arrowheads show single platelets, whereas the arrow in the ADAMTS13−/− mouse illustrates 3 platelets linearly arranged (scale bar=20 μm). MI indicates myocardial infarction.

Article Snippet: In Vivo Imaging Study Design Protocol 1 Closed-chest myocardial IR with 30 minutes of transient left anterior descending coronary artery (LAD) occlusion was performed in WT mice (n=40), WT mice treated with recombinant full-length human ADAMTS13, 5 μg IV (R&D Systems, Minneapolis, MN) just before IR (n=14), and ADAMTS13−/− mice (n=16).

Techniques: Control, Labeling, Immunostaining, Staining

Journal: Journal of cellular and molecular medicine

Article Title: The effect of ADAMTS13 on graft-versus-host disease.

doi: 10.1111/jcmm.18457

Figure Lengend Snippet: FIGURE 5 ADAMTS13 and VWF-A2 reduced donor-derived T cells in secondary lymphoid organs 24 h after bone marrow transplant. Mice were sacrificed 24 h post-transplant (n = 4). Cells were

Article Snippet: In some experiments, prior to adding Jurkat cells, histamine- stimulated HUVEC cells were treated with 1 μg/mL of recombinant human ADAMTS13 (6156- AD; R&D Systems) for 10 min at room temperature.

Techniques: Derivative Assay

Journal: Journal of cellular and molecular medicine

Article Title: The effect of ADAMTS13 on graft-versus-host disease.

doi: 10.1111/jcmm.18457

Figure Lengend Snippet: FIGURE 7 Role of VWF in the binding of T cells. (A) One hundred thousand Jurkat cells (immortalize malignant T lymphocytes) were incubated with HUVECs cells plated on 6-well plates. After 15 min incubation at 37°C, the number of adhered Jurkat cells in each well was counted using pictures taken with an inverted microscope and compared between histamine-stimulated and non-stimulated HUVECs. The effect of recombinant ADAMTS13 (1 mg/mL), recombinant VWF-A2 (1 mg/mL) and anti-αL antibodies (1:1000 dilution) on the number of adhered Jurkat cells to histamine- stimulated HUVECs were compared (n = 4, each experiment in triplicates). p-values were calculated using a one-way ANOVA test with Dunnett's multiple comparison correction compared to the number of adhered Jurkat cells to histamine-stimulated HUVECs. * p < 0.05. (B) One hundred thousand Jurkat cells were incubated in VWF-coated 96-wells plates for 15 min at 37°C. Jurkat cells, either pre-stimulated with 200 ng/mL of CCL21 or resting, were added to each well. The effect of recombinant ADAMTS13, recombinant VWF-A2 and anti-αL antibodies on the number of adhered CCL21-stimulated Jurkat cells to VWF was compared (n = 8, each experiment in triplicates). p-values were calculated using a one-way ANOVA test with Dunnett's multiple comparison correction compared to the number of adhered CCL21-stimulated Jurkat cells VWF. *p < 0.05.

Article Snippet: In some experiments, prior to adding Jurkat cells, histamine- stimulated HUVEC cells were treated with 1 μg/mL of recombinant human ADAMTS13 (6156- AD; R&D Systems) for 10 min at room temperature.

Techniques: Binding Assay, Incubation, Inverted Microscopy, Recombinant, Comparison